6 edition of Vital dyes in vitreoretinal surgery found in the catalog.
Vital dyes in vitreoretinal surgery
Includes bibliographical references and indexes.
|Statement||volume editor, Carsten H. Meyer.|
|Series||Developments in ophthalmology -- v. 42|
|Contributions||Meyer, Carsten H.|
|LC Classifications||RE501 .V56 2008|
|The Physical Object|
|LC Control Number||2008014732|
Bovine RPE cells therefore provide an easily available and cost-effective alternative, which does not entail ethical issues, unlike the establishment of human RPE cultures. Back of the Eye Disease and Treatment The vitreoretinal specialities address "Back of the Eye" diseases and treatments including surgery. Dr Andre Maia may be reached at maia retina. The aim of this article is to present the current data with regard to the application of vital dyes during vitreoretinal surgery, 'chromovitrectomy', as well as to overview the current literature regarding the properties of dyes, techniques of application, indications and complications in chromovitrectomy.
KG Karlsruhe, Germany. Likewise, the exposure of ARPE cells to ICG resulted in morphological alterations suggestive of cytoskeletal rearrangement and a decrease in viability. As a consequence, claim 1 related to two procedures, i. This study parts ways with past research which has suggested that a variety of dyes are appropriate for ophthalmic usage. Therefore, we consider trypan blue the best dye for staining the ERM.
The second exposure to the solvent or dye did not result in a remarkable difference in cellular morphology or the organization of the actin cytoskeleton that would suggest dedifferentiation. Identical staining of the retinal membrane and the adjacent tissue, which would inhibit differentiation, is thereby excluded. The background values of the negative control were subtracted from the absorbance values of all the test groups. Curr Eye Res.
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Therefore, the staining of ILM at the suggested concentrations could be applicable. The actin cytoskeleton was also arranged circumferentially underneath the plasma membrane. Conclusions Trisodium, Orangell and Methyl Violet can be applied in future studies in order to prove the capacity to stain preretinal tissues and vitreous without toxicity.
Indocyanine green-assisted peeling of the retinal internal limiting membrane. Grisanti recommended using isoosmolar ICG solutions with a concentration of 1.
Image analysis from one ex vivo study showed that staining increased linearly with dye concentration for some dyes, whereas others displayed an exponential relationship. Trypan blue staining of epiretinal membranes in proliferative vitreoretinopathy. Two oppositions were filed against the granted patent.
These patients had extreme cataracts—brunescent and black ones—and underwent cataract surgery. Vital dyes and light sources for chromovitrectomy: comparative assessment of osmolarity, pH, and spectrophotometry.
Indocyanine green ICG and infracyanine green may be considered the gold standard dyes for staining and visualizing the ILM in surgical therapy for macular hole and diabetic macular edema. Sheppard has Vital dyes in vitreoretinal surgery book financial interests related to his comments.
Table 1 Dye solutions, osmolarity measurements, and light absorbing properties of each dye tested Full size table Clinical Vital dyes in vitreoretinal surgery book with fundoscopy and biomicroscopy After intravitreal injection, MV, TR and OR appeared as a floating purple, green or orange mass in the vitreous.
Acta Ophthalmol. Modern laser-based in vivo imaging techniques such as intraoperative OCT can provide histological levels of detail, yet the limitations of human depth perception and hand-eye coordination mean that it remains a challenge for a surgeon to translate detailed imaging information into enhanced surgical performance.
Various types of robotic assistance could help propel intraocular surgery beyond these human physiological limitations. The comparison of potential and novel dye in an animal model is the main advantage of our study. The present invention may relate to a a single method comprising both staining of the retinal membrane and its removal; Vital dyes in vitreoretinal surgery book b a first method of staining the retinal membrane followed by a separate method of its removal.
As control, a group of cells were treated with low-serum medium in the same manner. It is correct that example 2 of the contested patent describes such a staining technique, which is, however, as correctly pointed out by the respondent, a preferred embodiment of the invention.
Different vital dyes has been tested for chromovitrectomy including trypan blue, patent blue, triamcinolone acetonide, infracyanine green, sodium fluorescein and brilliant blue.
More recently the use of dyes has been applied to stain perfluorcarbon liquids that may enhance its visualization during vitrectomy. In ophthalmology, vital dyes have become effective and useful surgical tools for identifying ocular tissues.
In this rabbit-model study, the retinal biocompatibility of MV, TR and OR were determined to evaluate these dyes as alternatives in chromovitrectomy. This dye may be used without fluid-air exchange; additionally, no dilution in glucose is necessary.
This decrease became significant with 0. No other adverse effects were observed in our study. This revolutionized vitreoretinal surgical techniques and made it possible to treat complex retinal detachments, vitreous haemorrhage, epiretinal membranes and macular holes.
Subretinal trypan blue migration during epiretinal membrane peeling. The extent of cellular junction formation and differentiation was evaluated by performing immunofluorescence staining for ZO-1, CTNNB1, and KRT19 as described later, and the results are demonstrated in Figure 1.
The animals in group 1 received Trisodium in the dose of 0.This pragmatic book provides an understanding of vitreoretinal surgery so important to the everyday practice.
Vital dyes in vitreoretinal surgery: chromovitrectomy / volume editor, Carsten H. Meyer - Details - Trove Trove: Find and get Australian resources. Books. Services provided by Dr Matthew Simunovic, vitreoretinal surgeon and medical retina specialist in Sydney.
Includes cataract surgery, macular surgery and laser eye surgery. (ILM). Visualisation of the ILM is enhanced with vital dyes such as ILM blue.
Gas is injected into the eye at the completion of surgery and is reabsorbed over a 2 week. Jun 30, · Since the advent of pars plana vitrectomy, immense technical advances in the treatment of retinal diseases have been made.
However, some mechanically based limitations of vitreoretinal surgery may result in incomplete removal of the vitreous, especially at the vitreoretinal interface and at the vitreous base.The use of intraocular vital dyes to enhance tissue visibility during retinal surgery pdf the potential to improve surgical performance and reduce risk.
The ideal retinal vital dye for selective membrane staining during vitreoretinal surgery should be water soluble, reasonably priced, and provide good color contrast, effective staining, and safety.Imaging techniques of the posterior segment of the eye download pdf gradually evolved and tremendously improved during the last decade.
A widespread implementation of optical coherence tomography (OCT) for the management and diagnosis of retinal conditions, with a concurrent advance in integrative technology, led to the integration of the OCT into the microscope for its intraoperative tjarrodbonta.com: Jesus Hernan Gonzalez-Cortes, Abraham Olvera-Barrios, Jesus Emiliano Gonzalez-Cantu, Jesus Mohamed-H.This pragmatic book ebook an understanding of vitreoretinal surgery so important to the everyday practice.
Vital dyes in vitreoretinal surgery: chromovitrectomy / volume editor, Carsten H. Meyer - Details - Trove Trove: Find and get Australian resources. Books.